gibson assembly cloning kit (New England Biolabs)
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New England Biolabs
gibson assembly cloning kit
Gibson Assembly Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4054 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gibson+assembly+kit/Gibson+Assembly+Cloning/10__1091_slash_mbc__e26___03___0143-254-12-11
Average 99 stars, based on 4054 article reviews
Gibson Assembly Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4054 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gibson+assembly+kit/Gibson+Assembly+Cloning/10__1091_slash_mbc__e26___03___0143-254-12-11
Average 99 stars, based on 4054 article reviews
gibson assembly cloning kit - by Bioz Stars,
2026-09
99/100 stars
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Polymerase Chain Reaction:Article Title: Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli ‐Based Tri‐Selection Article Snippet: .. First, error‐prone PCR kits from two different vendors GeneMorph II Random Mutagenesis Kit (Agilent, CA, USA), Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, CA, USA) were used to amplify the SsdA tox fragment, which replaced the WT SsdA tox fragment using the Article Title: Engineering Compact Base Editors by AlphaFold-Guided Mutation Scan and Escherichia coli-Based Tri-Selection. Article Snippet: .. First, error-prone PCR kits from two different vendors GeneMorph II Random Mutagenesis Kit (Agilent, CA, USA), Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, CA, USA) were used to amplify the SsdAtox fragment, which replaced the WT SsdAtox fragment using the Mutagenesis:Article Title: Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli ‐Based Tri‐Selection Article Snippet: .. First, error‐prone PCR kits from two different vendors GeneMorph II Random Mutagenesis Kit (Agilent, CA, USA), Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, CA, USA) were used to amplify the SsdA tox fragment, which replaced the WT SsdA tox fragment using the Article Title: Engineering Compact Base Editors by AlphaFold-Guided Mutation Scan and Escherichia coli-Based Tri-Selection. Article Snippet: .. First, error-prone PCR kits from two different vendors GeneMorph II Random Mutagenesis Kit (Agilent, CA, USA), Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, CA, USA) were used to amplify the SsdAtox fragment, which replaced the WT SsdAtox fragment using the Cloning:Article Title: Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli ‐Based Tri‐Selection Article Snippet: .. First, error‐prone PCR kits from two different vendors GeneMorph II Random Mutagenesis Kit (Agilent, CA, USA), Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, CA, USA) were used to amplify the SsdA tox fragment, which replaced the WT SsdA tox fragment using the Article Title: Engineering Compact Base Editors by AlphaFold-Guided Mutation Scan and Escherichia coli-Based Tri-Selection. Article Snippet: .. First, error-prone PCR kits from two different vendors GeneMorph II Random Mutagenesis Kit (Agilent, CA, USA), Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, CA, USA) were used to amplify the SsdAtox fragment, which replaced the WT SsdAtox fragment using the Article Title: Engineering Compact Base Editors by AlphaFold-Guided Mutation Scan and Escherichia coli-Based Tri-Selection. Article Snippet: .. To minimize the cytotoxic effects of SsdAtox in E. coli , a lowcopy-number plasmid (pBLC) used in our previous study [ 46 ] featuring the p15A origin was employed for cloning. (High-copynumber plasmids were unsuitable due to poor cloning efficiency.) pBLC-SsdACBE (Figure S1a ) was constructed by cloning SsdAtox sequence (Kind gift from Prof. Yongsub Kim [ 34 ]) in a base editor format (bpNLS-SsdA-nCas9-UGIx2-bpNLS) as a substitution for Cas9 in the pBLC-Cas9 vector [ 46 ] using the Plasmid Preparation:Article Title: Engineering Compact Base Editors by AlphaFold-Guided Mutation Scan and Escherichia coli-Based Tri-Selection. Article Snippet: .. To minimize the cytotoxic effects of SsdAtox in E. coli , a lowcopy-number plasmid (pBLC) used in our previous study [ 46 ] featuring the p15A origin was employed for cloning. (High-copynumber plasmids were unsuitable due to poor cloning efficiency.) pBLC-SsdACBE (Figure S1a ) was constructed by cloning SsdAtox sequence (Kind gift from Prof. Yongsub Kim [ 34 ]) in a base editor format (bpNLS-SsdA-nCas9-UGIx2-bpNLS) as a substitution for Cas9 in the pBLC-Cas9 vector [ 46 ] using the Construct:Article Title: Engineering Compact Base Editors by AlphaFold-Guided Mutation Scan and Escherichia coli-Based Tri-Selection. Article Snippet: .. To minimize the cytotoxic effects of SsdAtox in E. coli , a lowcopy-number plasmid (pBLC) used in our previous study [ 46 ] featuring the p15A origin was employed for cloning. (High-copynumber plasmids were unsuitable due to poor cloning efficiency.) pBLC-SsdACBE (Figure S1a ) was constructed by cloning SsdAtox sequence (Kind gift from Prof. Yongsub Kim [ 34 ]) in a base editor format (bpNLS-SsdA-nCas9-UGIx2-bpNLS) as a substitution for Cas9 in the pBLC-Cas9 vector [ 46 ] using the Sequencing:Article Title: Engineering Compact Base Editors by AlphaFold-Guided Mutation Scan and Escherichia coli-Based Tri-Selection. Article Snippet: .. To minimize the cytotoxic effects of SsdAtox in E. coli , a lowcopy-number plasmid (pBLC) used in our previous study [ 46 ] featuring the p15A origin was employed for cloning. (High-copynumber plasmids were unsuitable due to poor cloning efficiency.) pBLC-SsdACBE (Figure S1a ) was constructed by cloning SsdAtox sequence (Kind gift from Prof. Yongsub Kim [ 34 ]) in a base editor format (bpNLS-SsdA-nCas9-UGIx2-bpNLS) as a substitution for Cas9 in the pBLC-Cas9 vector [ 46 ] using the other:Article Title: NAD + sensing by PARP7 regulates the C/EBPβ-dependent transcription program during adipogenesis Article Snippet: Clone Assay:Article Title: NAD + sensing by PARP7 regulates the C/EBPβ-dependent transcription program during adipogenesis Article Snippet: .. The cDNAs were cloned into NheI - and XhoI -digested pInducer20 (Addgene, 44012; RRID:Addgene_44012) using a |